pūrere iti-utu engineered hoki 1 haora Covid kē whakamātautau

Kua apiti Harvard me MIT kairangahau ki runga ki te tauira i te whakamātautau hāngai huware-whaiaro-roto tātaritanga Covid e taea te wehewehe i waenganui i rerekē i roto i te haora, me te he "tika rite tika rite te PCR whakamātautau whakamahia inaianei", rite ki MIT.

Kei te karanga a te reira 'miSherlock', mō te 'minimally-instrumented Sherlock', i reira Sherlock ko te whakamātautau DNA / RNA Crispr-e hāngai ana whakaaro i a Harvard Wyss Institute hoki faauruhia biologically Engineering.

E hiahiatia e rua nga wahi: matū tākai-mua me te pūmārō mati arā, o ngā wāhanga hiko noa a 3D wahi tāngia. I te tihi o tenei, i reira ko te taupānga waea kōwhiringa.

E ai ki te Wyss Institute, e taea te huihui i te pūrere mō ~ $ 15 (~ $ 3 papatipu-hua), a kia utu whakamātautau a tawhio noa $ 6 ia.

"Ko e taha o nga mea nunui e pā ana ki miSherlock he e te reira katoa modular," ka mea a MIT kairangahau Devora Najjar. "Ko te pūrere iho he wehe i te assays, kia taea e koe te mono i roto i te rerekē assays mo te raupapa motuhake o RNA DNA ranei e ngana ana koe ki te kitea. Ka taea te hanga assays mō ngā whāinga hou i roto i pā ana ki te rua wiki, taea te whanaketanga o whakamātautau mō rerekē hou o te mahaha-ngaahi-2 rite me hoki atu ngā mate hopuhopu. "

He maha ngā wāhanga ki te anga matou matū (kite i raro), ka kua te pūmārō kua hangaia ki te tahuri enei ki takahanga ohie mo te kaiwhakamahi, e te tuwha atu tuatahi ki te ipu ritenga i roto i te kōrere iti tuku.

TKei te panaia ia huware ipu ki te tuatahi o rua ruma i roto i te taputapu pākahiko-Powered, te wahi kua oti te tahu ki te tauira te ki 95 ° C mō te toru ki te ono meneti i tauhohe reira me soaks ki te tātari.

Ko tenei tātari e haere ki te ruma tuarua, pana i te taha o te kaiwhakamahi ki te plunger e punctures hoki he awa ki te wai, ka meinga maroke matū Sherlock ki tauhohe ki te matūriki i runga i te mata o te tātari. Ka taea te mahi e rave rahi whakamātautau i roto i te ruma tuarua i te wa ano.

He i roto i-hanga whakahohe LED 55 meneti i muri mai ai te wai ki te fluoresce kite he, ki te roto i te reira Covid-19 ranei te kē motuhake o Covid-19 rapu i te mōkihi matū. Ko te kaiwhakamahi "taea hoki te whakamahi i te taupānga atamai tahi e tātari te pika i rēhita e kāmera o te atamai ki te whakarato i te pai mārama tātaritanga kino ranei," rite ki te Wyss Institute.

Ko te bio-matū

Sherlock (tauwhāiti tūtohutanga teitei enzymatic iritiraa kaipūrongo) whakamahi mahi 'Scissor rāpoi' a Crispr ki Katikati DNA RNA ranei i te wāhi motuhake, ka e he mahi tuarua ki te te tuatahi ko angitu

Hoki tenei whakamātautau, i tuatahi hangaia he tauhohenga Sherlock rite te whakamātautau paraikete ki te tautuhi i te tuhaa o Covid-19 RNA ngā i rerekē maha, a ka ki te meinga i te fluorescence ki te i angitu e.

hangaia muri tauhohe Sherlock i ki kite Arepa, Beta me Gamma (ko onge i roto i te kaupapa Delta) irakētanga i roto i te Covid-19 'matau' pūmua motuhake, rite tohu i tetahi rapunga ki fluorescence.

Ko te huware ngāwari ki te kohikohi whakaritea ki swabs ihu, e te aha i whiriwhiria ai, kia rite ki te Institute, engari ano hoki he huware whākōkī e meinga pai teka ki te kia tohua.

Te taahiraa whakawera whakahohetia matū i roto i te tauira ipu e aukati i enei whākōkī teka-pai, kia rite ki te pai kia rite ki ngā matū e whati whakatuwhera tetahi Covid-19 matūriki huaketo kia taea te mau i taua RNA huaketo i runga i te mata o te tātari rawa-hangaia.

Whakamatauria i runga i 27 Covid-19 tūroro me 21 tūroro hauora, kia rite ki a Harvard, tāutuhia te putanga paraikete o miSherlock 96% o Covid-19 tūroro me 95% o tūroro hauora. mahi te putanga Arepa, Beta me Gamma te rerekētanga o te whakamātautau i runga i ngā tauira horihori hanga i huware hauora, me te waihanga RNA viral i te whānuitanga o ngā nohanga.

Ko te Misherlock e whakaahuatia ana i roto i te 'Miihini Whakatakotoranga (Misherlock) mo te mate-a-ringa-a-ringa o te SSA-Cov-2 me nga rereketanga puta noa', whakaputaina i te ahunga whakamua, me te tirotiro me te kore e utu.